↓ Skip to main content

HIV Protocols

Overview of attention for book
Cover of 'HIV Protocols'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Quantifying CD4/CCR5 Usage Efficiency of HIV-1 Env Using the Affinofile System
  3. Altmetric Badge
    Chapter 2 Measuring T Cell-to-T Cell HIV-1 Transfer, Viral Fusion, and Infection Using Flow Cytometry
  4. Altmetric Badge
    Chapter 3 HIV-1 Capsid Stabilization Assay
  5. Altmetric Badge
    Chapter 4 Detection and Tracking of Dual-Labeled HIV Particles Using Wide-Field Live Cell Imaging to Follow Viral Core Integrity
  6. Altmetric Badge
    Chapter 5 HIV-1 Reverse Transcriptase-Based Assay to Determine Cellular dNTP Concentrations
  7. Altmetric Badge
    Chapter 6 Rapid Determination of HIV-1 Mutant Frequencies and Mutation Spectra Using an mCherry/EGFP Dual-Reporter Viral Vector
  8. Altmetric Badge
    Chapter 7 Novel Biochemical Tools for Probing HIV RNA Structure
  9. Altmetric Badge
    Chapter 8 Analysis of HIV-1 Gag-RNA Interactions in Cells and Virions by CLIP-seq.
  10. Altmetric Badge
    Chapter 9 Isolation of Cognate Cellular and Viral Ribonucleoprotein Complexes of HIV-1 RNA Applicable to Proteomic Discovery and Molecular Investigations
  11. Altmetric Badge
    Chapter 10 Methods for the Analyses of Inhibitor-Induced Aberrant Multimerization of HIV-1 Integrase
  12. Altmetric Badge
    Chapter 11 Quantification of HIV-1 Gag Localization Within Virus Producer Cells
  13. Altmetric Badge
    Chapter 12 Methods to Study Determinants for Membrane Targeting of HIV-1 Gag In Vitro
  14. Altmetric Badge
    Chapter 13 Visualizing the Behavior of HIV-Infected T Cells In Vivo Using Multiphoton Intravital Microscopy
  15. Altmetric Badge
    Chapter 14 Modeling HIV-1 Mucosal Transmission and Prevention in Humanized Mice
  16. Altmetric Badge
    Chapter 15 High-Throughput Humanized Mouse Models for Evaluation of HIV-1 Therapeutics and Pathogenesis
  17. Altmetric Badge
    Chapter 16 Measuring the Frequency of Latent HIV-1 in Resting CD4 + T Cells Using a Limiting Dilution Coculture Assay
  18. Altmetric Badge
    Chapter 17 LGIT In Vitro Latency Model in Primary and T Cell Lines to Test HIV-1 Reactivation Compounds
  19. Altmetric Badge
    Chapter 18 Improved Methods to Detect Low Levels of HIV Using Antibody-Based Technologies
  20. Altmetric Badge
    Chapter 19 Analysis of ABCA1 and Cholesterol Efflux in HIV-Infected Cells
  21. Altmetric Badge
    Chapter 20 The Proteomic Characterization of Plasma or Serum from HIV-Infected Patients
  22. Altmetric Badge
    Chapter 21 Proteomic Characterization of Exosomes from HIV-1-Infected Cells
  23. Altmetric Badge
    Chapter 22 Detecting HIV-1 Tat in Cell Culture Supernatants by ELISA or Western Blot
  24. Altmetric Badge
    Chapter 23 Protocol for Detection of HIV-Tat Protein in Cerebrospinal Fluid by a Sandwich Enzyme-Linked Immunosorbent Assay
  25. Altmetric Badge
    Chapter 24 Measuring the Uptake and Transactivation Function of HIV-1 Tat Protein in a Trans-cellular Cocultivation Setup.
  26. Altmetric Badge
    Chapter 25 Evaluating the Role of Viral Proteins in HIV-Mediated Neurotoxicity Using Primary Human Neuronal Cultures.
  27. Altmetric Badge
    Chapter 26 Erratum
Attention for Chapter 19: Analysis of ABCA1 and Cholesterol Efflux in HIV-Infected Cells
Altmetric Badge

Citations

dimensions_citation
3 Dimensions

Readers on

mendeley
9 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
Analysis of ABCA1 and Cholesterol Efflux in HIV-Infected Cells
Chapter number 19
Book title
HIV Protocols
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3046-3_19
Pubmed ID
Book ISBNs
978-1-4939-3045-6, 978-1-4939-3046-3
Authors

Nigora Mukhamedova, Beda Brichacek, Christina Darwish, Anastas Popratiloff, Dmitri Sviridov, Michael Bukrinsky

Abstract

Cholesterol is an essential component of the cellular membranes and, by extension, of the HIV envelope membrane, which is derived from the host cell plasma membrane. Depletion of the cellular cholesterol has an inhibitory effect on HIV assembly, reduces infectivity of the produced virions, and makes the cell less susceptible to HIV infection. It is not surprising that the virus has evolved to gain access to cellular proteins regulating cholesterol metabolism. One of the key mechanisms used by HIV to maintain high levels of cholesterol in infected cells is Nef-mediated inhibition of cholesterol efflux and the cholesterol transporter responsible for this process, ABCA1. In this chapter, we describe methods to investigate these effects of HIV-1 infection.

Mendeley readers

The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 9 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 2 22%
Professor 1 11%
Student > Bachelor 1 11%
Student > Ph. D. Student 1 11%
Other 1 11%
Other 1 11%
Unknown 2 22%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 44%
Agricultural and Biological Sciences 1 11%
Immunology and Microbiology 1 11%
Neuroscience 1 11%
Unknown 2 22%