Chapter title |
Modeling HIV-1 Mucosal Transmission and Prevention in Humanized Mice
|
---|---|
Chapter number | 14 |
Book title |
HIV Protocols
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3046-3_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3045-6, 978-1-4939-3046-3
|
Authors |
Milena Veselinovic, Paige Charlins, Ramesh Akkina, Veselinovic, Milena, Charlins, Paige, Akkina, Ramesh |
Abstract |
The new generation humanized mice (hu-mice) that permit continuous de novo generation of human hematopoietic cells have led to novel strategies in studying HIV-1 pathogenesis, prevention and therapies. HIV-1 infection of hu-mice results in chronic viremia and CD4+ T cell loss, thus mimicking key aspects of the disease progression. In addition, the new generation hu-mice are permissive for HIV-1 sexual transmission by vaginal and rectal routes thus allowing in vivo efficacy testing of new anti-HIV-1 drugs for prevention. Two leading models are currently being used, namely the hu-HSC mice and the BLT mice. Here we describe the methodology for generating both hu-HSC and BLT mice and their use in the study of HIV-1 transmission and prevention of infection by topical and oral administration of anti-retroviral drugs. Practical aspects of the methodologies are emphasized. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Canada | 1 | 4% |
Brazil | 1 | 4% |
Unknown | 22 | 92% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 5 | 21% |
Student > Bachelor | 4 | 17% |
Professor | 1 | 4% |
Other | 1 | 4% |
Student > Ph. D. Student | 1 | 4% |
Other | 1 | 4% |
Unknown | 11 | 46% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 5 | 21% |
Immunology and Microbiology | 3 | 13% |
Biochemistry, Genetics and Molecular Biology | 1 | 4% |
Nursing and Health Professions | 1 | 4% |
Agricultural and Biological Sciences | 1 | 4% |
Other | 2 | 8% |
Unknown | 11 | 46% |