Chapter title |
Assessing PTEN Subcellular Localization
|
---|---|
Chapter number | 12 |
Book title |
PTEN
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3299-3_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3297-9, 978-1-4939-3299-3
|
Authors |
Anabel Gil, José I. López, Rafael Pulido |
Abstract |
PTEN subcellular localization is fundamental in the execution of the distinct PTEN biological activities, including not only its PI(3,4,5)P3 phosphatase activity when associated to membranes but also its subcellular compartment-specific interactions with regulatory and effector proteins, including those exerted in the nucleus. As a consequence, PTEN subcellular localization is tightly regulated in vivo by both intrinsic and extrinsic mechanisms. The plasma membrane/nucleus/cytoplasm partitioning of PTEN has been the focus of several studies, both from a mechanistic and from a disease-association point of view. Here, we summarize the current knowledge on PTEN plasma membrane/nucleus/cytoplasm distribution, and present subcellular fractionation, immunofluorescence, and immunohistochemical methods to study the distribution and shuttling of PTEN between these subcellular compartments. |
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