| Chapter title |
Applications of Yeast Surface Display for Protein Engineering.
|
|---|---|
| Chapter number | 8 |
| Book title |
Yeast Surface Display
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2748-7_8 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2747-0, 978-1-4939-2748-7
|
| Authors |
Cherf, Gerald M, Cochran, Jennifer R, Gerald M. Cherf, Jennifer R. Cochran, Cherf, Gerald M., Cochran, Jennifer R. |
| Abstract |
The method of displaying recombinant proteins on the surface of Saccharomyces cerevisiae via genetic fusion to an abundant cell wall protein, a technology known as yeast surface display, or simply, yeast display, has become a valuable protein engineering tool for a broad spectrum of biotechnology and biomedical applications. This review focuses on the use of yeast display for engineering protein affinity, stability, and enzymatic activity. Strategies and examples for each protein engineering goal are discussed. Additional applications of yeast display are also briefly presented, including protein epitope mapping, identification of protein-protein interactions, and uses of displayed proteins in industry and medicine. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Israel | 1 | <1% |
| United States | 1 | <1% |
| Germany | 1 | <1% |
| Brazil | 1 | <1% |
| Unknown | 418 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 105 | 25% |
| Student > Bachelor | 64 | 15% |
| Researcher | 52 | 12% |
| Student > Master | 38 | 9% |
| Student > Doctoral Student | 16 | 4% |
| Other | 40 | 9% |
| Unknown | 107 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 144 | 34% |
| Agricultural and Biological Sciences | 57 | 14% |
| Engineering | 25 | 6% |
| Chemistry | 22 | 5% |
| Chemical Engineering | 14 | 3% |
| Other | 49 | 12% |
| Unknown | 111 | 26% |