Chapter title |
Isolation and Validation of Anti-B7-H4 scFvs from an Ovarian Cancer scFv Yeast-Display Library.
|
---|---|
Chapter number | 2 |
Book title |
Yeast Surface Display
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2748-7_2 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2747-0, 978-1-4939-2748-7
|
Authors |
Dangaj, Denarda, Scholler, Nathalie, Denarda Dangaj, Nathalie Scholler |
Abstract |
B7-H4 (VTCN1, B7x, B7s) is an inhibitory modulator of T-cell response implicated in antigen tolerization. As such, B7-H4 is an immune checkpoint of potential therapeutic interest. To generate anti-B7-H4 targeting reagents, we isolated antibodies by differential cell screening of a yeast-display library of recombinant antibodies (scFvs) derived from ovarian cancer patients and we screened for functional scFvs capable to interfere with B7-H4-mediated inhibition of antitumor responses. We found one antibody binding to B7-H4 that could restore antitumor T cell responses. This chapter gives an overview of the methods we developed to isolate a functional anti-B7-H4 antibody fragment. |
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