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X Demographics
Mendeley readers
Attention Score in Context
| Chapter title |
Yeast Display-Based Antibody Affinity Maturation Using Detergent-Solubilized Cell Lysates.
|
|---|---|
| Chapter number | 4 |
| Book title |
Yeast Surface Display
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2748-7_4 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2747-0, 978-1-4939-2748-7
|
| Authors |
Tillotson, Benjamin J, Lajoie, Jason M, Shusta, Eric V, Benjamin J. Tillotson, Jason M. Lajoie, Eric V. Shusta, Tillotson, Benjamin J., Lajoie, Jason M., Shusta, Eric V. |
| Abstract |
It is often desired to identify or engineer antibodies that target membrane proteins (MPs). However, due to their inherent insolubility in aqueous solutions, MPs are often incompatible with in vitro antibody discovery and optimization platforms. Recently, we adapted yeast display technology to accommodate detergent-solubilized cell lysates as sources of MP antigens. The following protocol details the incorporation of cell lysates into a kinetic screen designed to obtain antibodies with improved affinity via slowed dissociation from an MP antigen. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 24 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 4% |
| Unknown | 23 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 6 | 25% |
| Researcher | 5 | 21% |
| Student > Master | 3 | 13% |
| Professor > Associate Professor | 2 | 8% |
| Student > Bachelor | 2 | 8% |
| Other | 2 | 8% |
| Unknown | 4 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 6 | 25% |
| Engineering | 4 | 17% |
| Agricultural and Biological Sciences | 4 | 17% |
| Chemical Engineering | 2 | 8% |
| Chemistry | 1 | 4% |
| Other | 1 | 4% |
| Unknown | 6 | 25% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 June 2015.
All research outputs
#20,278,422
of 22,811,321 outputs
Outputs from Methods in molecular biology
#9,909
of 13,119 outputs
Outputs of similar age
#295,824
of 353,098 outputs
Outputs of similar age from Methods in molecular biology
#635
of 996 outputs
Altmetric has tracked 22,811,321 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,119 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 353,098 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 996 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.