| Chapter title |
Elaboration and Physicochemical Characterization of Niosome-Based Nioplexes for Gene Delivery Purposes
|
|---|---|
| Chapter number | 5 |
| Book title |
Non-Viral Gene Delivery Vectors
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3718-9_5 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3716-5, 978-1-4939-3718-9
|
| Authors |
Edilberto Ojeda, Mireia Agirre, Ilia Villate-Beitia, Mohamed Mashal, Gustavo Puras, Jon Zarate, Jose L. Pedraz |
| Abstract |
Niosome formulations for gene delivery purposes are based on nonionic surfactants, helper lipids, and cationic lipids that interact electrostatically with negatively charged DNA molecules to form the so-called nioplexes. Niosomes are elaborated by different techniques, such as solvent emulsion-evaporation, thin film hydration, hand-shaking, dissolvent injection, and microfluidization method, among many others. In this chapter, we have described some protocols for the elaboration of niosomes and nioplexes and their physicochemical characterization that guarantees the quality criteria of the formulation in terms of size, morphology, ζ-potential, and stability. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 16 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 3 | 19% |
| Researcher | 3 | 19% |
| Librarian | 1 | 6% |
| Student > Doctoral Student | 1 | 6% |
| Other | 1 | 6% |
| Other | 2 | 13% |
| Unknown | 5 | 31% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 5 | 31% |
| Pharmacology, Toxicology and Pharmaceutical Science | 3 | 19% |
| Agricultural and Biological Sciences | 1 | 6% |
| Social Sciences | 1 | 6% |
| Unknown | 6 | 38% |