| Chapter title |
Anionic/Zwitterionic Lipid-Based Gene Vectors of pDNA
|
|---|---|
| Chapter number | 4 |
| Book title |
Non-Viral Gene Delivery Vectors
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3718-9_4 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3716-5, 978-1-4939-3718-9
|
| Authors |
Ana L. Barrán-Berdón, Emilio Aicart, Elena Junquera |
| Abstract |
The use of anionic lipids (ALs) as non-viral gene vectors depicts a promising alternative to cationic lipids (CLs) since they are more biocompatible and present lower levels of phagocytosis by macrophages. Several experimental methods, such as electrophoretic mobility (ζ-potential), gel electrophoresis, small-angle X-ray scattering (SAXS), fluorescence and confocal fluorescence microscopies (FM and CFM), flow assisted cell sorting-flow cytometry (FACS-FCM), and cell viability/cytotoxicity assays can be used for a complete physicochemical and biochemical characterization of lipoplexes formed by an AL, a zwitterionic lipid (ZL), and a plasmid DNA (pDNA), their electrostatic interaction being necessarily mediated by divalent cations, such as Ca(2+). In the present chapter, we summarize the protocols optimized for the mentioned characterization techniques. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 4 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 1 | 25% |
| Researcher | 1 | 25% |
| Lecturer | 1 | 25% |
| Unknown | 1 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 2 | 50% |
| Biochemistry, Genetics and Molecular Biology | 1 | 25% |
| Unknown | 1 | 25% |