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Molecular Genetics of Asthma

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Attention for Chapter 11: Protocols for Exosome Isolation and RNA Profiling
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Chapter title
Protocols for Exosome Isolation and RNA Profiling
Chapter number 11
Book title
Molecular Genetics of Asthma
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3652-6_11
Pubmed ID
Book ISBNs
978-1-4939-3650-2, 978-1-4939-3652-6
Authors

Fernando Marqués-García, María Isidoro-García, Marqués-García, Fernando, Isidoro-García, María

Abstract

Exosomes are small extracellular vesicles of multivesicular bodies derived from the cell endosome. Molecules have a characteristic composition both in the membrane and in the carried load. These particles are located in the culture medium of cells in vitro and in vivo body fluids, being synthesized by almost all kind of cells. The most characteristic molecule transported within is the RNA with a large clinical potential. In this chapter, the methodology for isolation of exosomes and subsequent purification of the RNA is reviewed.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 52 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 52 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 17 33%
Researcher 6 12%
Student > Bachelor 3 6%
Other 3 6%
Professor > Associate Professor 3 6%
Other 9 17%
Unknown 11 21%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 13 25%
Medicine and Dentistry 10 19%
Agricultural and Biological Sciences 9 17%
Engineering 2 4%
Chemistry 2 4%
Other 5 10%
Unknown 11 21%