Chapter title |
Molecular Genetics of Asthma
|
---|---|
Chapter number | 3 |
Book title |
Molecular Genetics of Asthma
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3652-6_3 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3650-2, 978-1-4939-3652-6
|
Authors |
Segundo-Val, Ignacio San, Sanz-Lozano, Catalina S, Ignacio San Segundo-Val, Catalina S. Sanz-Lozano, San Segundo-Val, Ignacio, Sanz-Lozano, Catalina S. |
Abstract |
In 1941, Beadle and Tatum published experiments that would explain the basis of the central dogma of molecular biology, whereby the DNA through an intermediate molecule, called RNA, results proteins that perform the functions in cells. Currently, biomedical research attempts to explain the mechanisms by which develops a particular disease, for this reason, gene expression studies have proven to be a great resource. Strictly, the term "gene expression" comprises from the gene activation until the mature protein is located in its corresponding compartment to perform its function and contribute to the expression of the phenotype of cell.The expression studies are directed to detect and quantify messenger RNA (mRNA) levels of a specific gene. The development of the RNA-based gene expression studies began with the Northern Blot by Alwine et al. in 1977. In 1969, Gall and Pardue and John et al. independently developed the in situ hybridization, but this technique was not employed to detect mRNA until 1986 by Coghlan. Today, many of the techniques for quantification of RNA are deprecated because other new techniques provide more information. Currently the most widely used techniques are qPCR, expression microarrays, and RNAseq for the transcriptome analysis. In this chapter, these techniques will be reviewed. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 4 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 3 | 75% |
Scientists | 1 | 25% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 292 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 49 | 17% |
Student > Master | 38 | 13% |
Student > Ph. D. Student | 31 | 11% |
Researcher | 16 | 5% |
Unspecified | 11 | 4% |
Other | 31 | 11% |
Unknown | 116 | 40% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 81 | 28% |
Agricultural and Biological Sciences | 31 | 11% |
Medicine and Dentistry | 17 | 6% |
Unspecified | 12 | 4% |
Immunology and Microbiology | 5 | 2% |
Other | 24 | 8% |
Unknown | 122 | 42% |