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Mendeley readers
| Chapter title |
Protocol for Lipid-Mediated Transient Transfection in A549 Epithelial Lung Cell Line
|
|---|---|
| Chapter number | 13 |
| Book title |
Molecular Genetics of Asthma
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3652-6_13 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3650-2, 978-1-4939-3652-6
|
| Authors |
Elena Marcos-Vadillo, Asunción García-Sánchez, Marcos-Vadillo, Elena, García-Sánchez, Asunción |
| Abstract |
Trials of transfection in eukaryotic cells are essential tools for the study of gene and protein function. They have been used in a wide range of research fields. In this chapter, a method of transient transfection of the A549 cell line, human lung cells of alveolar epithelium, with an expression plasmid is described. In addition, the fundamental characteristics of this experimental procedure are addressed. |
Mendeley readers
The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 16 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 6 | 38% |
| Student > Master | 4 | 25% |
| Researcher | 2 | 13% |
| Student > Doctoral Student | 1 | 6% |
| Student > Bachelor | 1 | 6% |
| Other | 0 | 0% |
| Unknown | 2 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 9 | 56% |
| Medicine and Dentistry | 2 | 13% |
| Immunology and Microbiology | 1 | 6% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 6% |
| Unknown | 3 | 19% |