Chapter title |
Intermolecular Paramagnetic Relaxation Enhancement (PRE) Studies of Transient Complexes in Intrinsically Disordered Proteins.
|
---|---|
Chapter number | 3 |
Book title |
Protein Amyloid Aggregation
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-2978-8_3 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2977-1, 978-1-4939-2978-8
|
Authors |
Janowska, Maria K, Baum, Jean, Maria K. Janowska, Jean Baum |
Abstract |
NMR interchain paramagnetic relaxation enhancement (PRE) techniques are a very powerful approach for detecting transient interchain interactions between intrinsically disordered proteins. These experiments, requiring a mixed sample containing a 1:1 ratio of isotope-labeled (15)N protein and natural abundance (14)N protein with a paramagnetic spin label, provide data that is limited to interchain interactions only. Application of these experiments to weakly associated transient species such as those that are present in the very early stages of self-assembly processes will aid our understanding of protein aggregation or fibril formation processes. |
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