Chapter title |
A qRT-PCR Method for Determining the Biodistribution Profile of a miR-34a Mimic.
|
---|---|
Chapter number | 8 |
Book title |
Gene Therapy of Solid Cancers
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2727-2_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2726-5, 978-1-4939-2727-2
|
Authors |
Kelnar, Kevin, Bader, Andreas G, Kevin Kelnar, Andreas G. Bader, Bader, Andreas G. |
Abstract |
MRX34 has recently entered the clinic as the first therapeutic product based on a microRNA (miRNA) mimic. In order to measure drug concentrations in vivo, a quantitation method is needed that exhibits high precision, accuracy, and robustness. While most clinical applications for oligonucleotide therapeutics involve methods based on hybridization assays and liquid chromatography-tandem mass spectrometry, quantitative PCR (qPCR) is a less well-described approach. Here, we present an RT, qPCR, and analysis method to determine the tissue biodistribution of endogenous as well as a therapeutic, exogenous miRNA mimic therapeutic. Assay performance is demonstrated on multiple tissues from nonhuman primates dosed with MRX34. |
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