Chapter title |
Chromatin Immunoprecipitation Assay: Examining the Interaction of NFkB with the VEGF Promoter.
|
---|---|
Chapter number | 6 |
Book title |
VEGF Signaling
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2917-7_6 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2916-0, 978-1-4939-2917-7
|
Authors |
Walton, Chad B, Matter, Michelle L, Chad B. Walton, Michelle L. Matter, Walton, Chad B., Matter, Michelle L. |
Abstract |
The chromatin immunoprecipitation (ChIP) assay is a versatile technique used to evaluate the association of proteins with specific DNA regions both in vivo and in vitro. This assay can be used to identify proteins associated with a specific region of the genome, or the opposite, to identify the many regions of the genome associated with a particular protein. The ChIP assay can also be used to analyze binding of transcription factors, transcription cofactors, DNA replication factors, and DNA repair proteins. Here we describe a useful ChIP-qPCR protocol to examine the interaction of NFkB with the VEGF promoter in adult rat primary cardiomyocytes that have been mechanically stretched after attaching to the extracellular matrix protein laminin. |
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