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Stem Cells and Good Manufacturing Practices

Overview of attention for book
Cover of 'Stem Cells and Good Manufacturing Practices'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 100 Scalable Ex Vivo Expansion of Human Mesenchymal Stem/Stromal Cells in Microcarrier-Based Stirred Culture Systems
  3. Altmetric Badge
    Chapter 101 GMP-Grade Human Fetal Liver-Derived Mesenchymal Stem Cells for Clinical Transplantation
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    Chapter 102 Culture of Human Limbal Epithelial Stem Cells on Tenon’s Fibroblast Feeder-Layers: A Translational Approach
  5. Altmetric Badge
    Chapter 103 Standard Operating Procedure for the Good Manufacturing Practice-Compliant Production of Human Bone Marrow Mesenchymal Stem Cells
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    Chapter 104 Media Fill for Validation of a Good Manufacturing Practice-Compliant Cell Production Process
  7. Altmetric Badge
    Chapter 105 cGMP-Compliant Transportation Conditions for a Prompt Therapeutic Use of Marrow Mesenchymal Stromal/Stem Cells
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    Chapter 106 Scalable Expansion of Human-Induced Pluripotent Stem Cells in Xeno-Free Microcarriers
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    Chapter 112 GMP-Compliant Human Adipose Tissue-Derived Mesenchymal Stem Cells for Cellular Therapy
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    Chapter 115 Purification of Human Induced Pluripotent Stem Cell-Derived Neural Precursors Using Magnetic Activated Cell Sorting
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    Chapter 116 Isolation and Expansion of Mesenchymal Stromal/Stem Cells from Umbilical Cord Under Chemically Defined Conditions
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    Chapter 117 Bioreactor Expansion of Human Mesenchymal Stem Cells According to GMP Requirements
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    Chapter 118 The Suspension Culture of Undifferentiated Human Pluripotent Stem Cells Using Spinner Flasks
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    Chapter 122 Xeno-Free Culture of Human Periodontal Ligament Stem Cells.
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    Chapter 123 Labeling Pluripotent Stem Cell-Derived Neural Progenitors with Iron Oxide Particles for Magnetic Resonance Imaging.
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    Chapter 124 Derivation of GMP-Compliant Integration-Free hiPSCs Using Modified mRNAs
  17. Altmetric Badge
    Chapter 125 Production of Good Manufacturing Practice-Grade Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells for Therapeutic Use
  18. Altmetric Badge
    Chapter 164 Using the Quantum Cell Expansion System for the Automated Expansion of Clinical-Grade Bone Marrow-Derived Human Mesenchymal Stromal Cells
  19. Altmetric Badge
    Chapter 175 Therapeutic Mesenchymal Stromal Cells: Where We Are Headed
Attention for Chapter 122: Xeno-Free Culture of Human Periodontal Ligament Stem Cells.
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Chapter title
Xeno-Free Culture of Human Periodontal Ligament Stem Cells.
Chapter number 122
Book title
Stem Cells and Good Manufacturing Practices
Published in
Methods in molecular biology, October 2014
DOI 10.1007/7651_2014_122
Pubmed ID
25326670
Book ISBNs
978-1-4939-2434-9, 978-1-4939-2435-6
Authors

Oriana Trubiani, Francesca Diomede, Trubiani, Oriana, Diomede, Francesca

Abstract

The possibility of transplanting adult stem cells into damaged organs has opened a new prospective for the treatment of several human pathologies. Currently, in vitro expansion and culture of mesenchymal stem cells is founded on supplementing cell culture and differentiation medium with fetal calf serum (FCS) or fetal bovine serum (FBS) that contain numerous growth factors inducing cell attachment to plastic surfaces, proliferation, and differentiation. Mesenchymal stem cells (MSCs) cultured with medium containing FCS or FBS are unusable in the cell therapy; in fact the central issues regarding limitations in using animal sera for cell therapy is that its components are highly variable and often unknown and may trigger a xenogenic immune response, immunological reactions, and the potential transmission of prion diseases and zoonoses.Here we describe the culture system protocols for the expansion and production of human Periodontal Ligament Stem Cells (hPDLSCs) using a new xeno-free medium formulation ensuring the maintenance of the stem cells features comprising the multiple passage expansion, mesengenic lineage differentiation, cellular phenotype, and genomic stability, essential elements for conforming to translation to cell therapy.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 7%
Australia 1 7%
Unknown 13 87%

Demographic breakdown

Readers by professional status Count As %
Student > Master 4 27%
Researcher 2 13%
Unspecified 1 7%
Student > Ph. D. Student 1 7%
Student > Bachelor 1 7%
Other 2 13%
Unknown 4 27%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 20%
Chemical Engineering 2 13%
Psychology 2 13%
Agricultural and Biological Sciences 1 7%
Unspecified 1 7%
Other 2 13%
Unknown 4 27%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 October 2014.
All research outputs
#20,241,019
of 22,768,097 outputs
Outputs from Methods in molecular biology
#9,865
of 13,090 outputs
Outputs of similar age
#215,762
of 258,576 outputs
Outputs of similar age from Methods in molecular biology
#92
of 126 outputs
Altmetric has tracked 22,768,097 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,090 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 258,576 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 126 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.

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