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Chromosome and Genomic Engineering in Plants

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Cover of 'Chromosome and Genomic Engineering in Plants'

Table of Contents

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    Book Overview
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    Chapter 1 Production of Engineered Minichromosome Vectors via the Introduction of Telomere Sequences
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    Chapter 2 Method for Biolistic Site-Specific Integration in Plants Catalyzed by Bxb1 Integrase
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    Chapter 3 Protocol for In Vitro Stacked Molecules Compatible with In Vivo Recombinase-Mediated Gene Stacking
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    Chapter 4 Chromosome and Genomic Engineering in Plants
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    Chapter 5 One-Step Generation of Chromosomal Rearrangements in Rice
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    Chapter 6 Genome Elimination by Tailswap CenH3: In Vivo Haploid Production in Arabidopsis thaliana
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    Chapter 7 Chromosome and Genomic Engineering in Plants
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    Chapter 8 CRISPR/Cas-Mediated Site-Specific Mutagenesis in Arabidopsis thaliana Using Cas9 Nucleases and Paired Nickases
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    Chapter 9 Chromosome and Genomic Engineering in Plants
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    Chapter 10 Seamless Genome Editing in Rice via Gene Targeting and Precise Marker Elimination
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    Chapter 11 Chromosome and Genomic Engineering in Plants
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    Chapter 12 Chromosome and Genomic Engineering in Plants
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    Chapter 13 Image Analysis of DNA Fiber and Nucleus in Plants
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    Chapter 14 Chromosome and Genomic Engineering in Plants
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    Chapter 15 Chromosome and Genomic Engineering in Plants
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    Chapter 16 Chromosome and Genomic Engineering in Plants
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    Chapter 17 Mapping of T-DNA and Ac/Ds by TAIL-PCR to Analyze Chromosomal Rearrangements
Attention for Chapter 17: Mapping of T-DNA and Ac/Ds by TAIL-PCR to Analyze Chromosomal Rearrangements
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Chapter title
Mapping of T-DNA and Ac/Ds by TAIL-PCR to Analyze Chromosomal Rearrangements
Chapter number 17
Book title
Chromosome and Genomic Engineering in Plants
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-4931-1_17
Pubmed ID
Book ISBNs
978-1-4939-4929-8, 978-1-4939-4931-1
Authors

Satoru Fujimoto, Sachihiro Matsunaga, Minoru Murata

Abstract

Insertion mutagenesis using known DNA sequences such as T-DNA and transposons is an important tool for studies on gene function in plant sciences. The transposons Activator (Ac)/Dissociation (Ds) have been systematically used to manipulate plant chromosomes. For both of these applications, the recovery of genomic DNA sequences flanking the insertions is required to estimate the sizes and/or scales of the reconstituted chromosomes. In this chapter, we describe the protocols for thermal asymmetric interlaced PCR (TAIL-PCR) for isolation of genomic sequences flanking DNA inserts in plant genomes.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 2 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 2 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 1 50%
Unknown 1 50%
Readers by discipline Count As %
Agricultural and Biological Sciences 1 50%
Unknown 1 50%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 December 2017.
All research outputs
#18,581,651
of 23,015,156 outputs
Outputs from Methods in molecular biology
#7,962
of 13,156 outputs
Outputs of similar age
#285,659
of 394,707 outputs
Outputs of similar age from Methods in molecular biology
#845
of 1,471 outputs
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