| Chapter title |
Genome Elimination by Tailswap CenH3: In Vivo Haploid Production in Arabidopsis thaliana
|
|---|---|
| Chapter number | 6 |
| Book title |
Chromosome and Genomic Engineering in Plants
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-4931-1_6 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-4929-8, 978-1-4939-4931-1
|
| Authors |
Maruthachalam Ravi, Ramesh Bondada |
| Abstract |
Artificial production of haploids is one of the important sought-after goals of plant breeding and crop improvement programs. Conventionally, haploid plants are generated by in vitro (tissue) culture of haploid plant gametophytes, pollen (male), and embryo sac (female). Here, we describe a facile, nontissue culture-based in vivo method of haploid production through seeds in the model plant, Arabidopsis thaliana. This method involves simple crossing of any desired genotype of interest to a haploid-inducing strain (GFP-tailswap) to directly obtain haploid F1 seeds. The described protocol can be practiced by anyone with basic experience in growing A. thaliana plants and will be of interest to Arabidopsis research community. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 12 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 3 | 25% |
| Student > Doctoral Student | 2 | 17% |
| Student > Ph. D. Student | 1 | 8% |
| Student > Bachelor | 1 | 8% |
| Professor > Associate Professor | 1 | 8% |
| Other | 1 | 8% |
| Unknown | 3 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 5 | 42% |
| Biochemistry, Genetics and Molecular Biology | 3 | 25% |
| Earth and Planetary Sciences | 1 | 8% |
| Unknown | 3 | 25% |