Chapter title |
Engineering THIOMABs for Site-Specific Conjugation of Thiol-Reactive Linkers.
|
---|---|
Chapter number | 11 |
Book title |
Antibody-Drug Conjugates
|
Published in |
Methods in molecular biology, August 2013
|
DOI | 10.1007/978-1-62703-541-5_11 |
Pubmed ID | |
Book ISBNs |
978-1-62703-540-8, 978-1-62703-541-5
|
Authors |
Bhakta S, Raab H, Junutula JR, Sunil Bhakta, Helga Raab, Jagath R. Junutula, Bhakta, Sunil, Raab, Helga, Junutula, Jagath R. |
Abstract |
Antibody conjugates are used in many therapeutic and research applications and are generated by chemically linking a cysteine or lysine residue to potent chemotherapeutic drugs or other functional groups through a flexible linker. Recently, we have engineered THIOMABs (antibodies with engineered reactive cysteine residues) for site-specific conjugation and showed that these antibody conjugates display homogeneous labeling with optimal in vitro and in vivo characteristics. Here, we describe protocols for engineering, selection, and site-specific conjugation of THIOMABs with thiol-reactive linkers. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 54 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 14 | 26% |
Student > Master | 11 | 20% |
Student > Ph. D. Student | 11 | 20% |
Student > Bachelor | 3 | 6% |
Student > Doctoral Student | 2 | 4% |
Other | 5 | 9% |
Unknown | 8 | 15% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 14 | 26% |
Chemistry | 12 | 22% |
Agricultural and Biological Sciences | 8 | 15% |
Pharmacology, Toxicology and Pharmaceutical Science | 3 | 6% |
Veterinary Science and Veterinary Medicine | 2 | 4% |
Other | 5 | 9% |
Unknown | 10 | 19% |