Chapter 1 Variation in Salamanders: An Essay on Genomes, Development, and Evolution.

Chapter 2 Maintaining Eastern Newts ( Notophthalmus viridescens ) for Regeneration Research

Chapter 3 Housing and Maintenance of Ambystoma mexicanum, the Mexican Axolotl

Chapter 4 Husbandry of Spanish Ribbed Newts ( Pleurodeles waltl )

Chapter 5 Maintaining Plethodontid Salamanders in the Laboratory for Regeneration Studies

Chapter 6 Newt Lens Transdifferentiation: From Lentectomy to Immuno-FISH.

Chapter 7 Studying Newt Brain Regeneration Following Subtype Specific Neuronal Ablation

Chapter 8 The Accessory Limb Model: An Alternative Experimental System of Limb Regeneration

Chapter 9 High-Efficiency Electroporation of the Spinal Cord in Larval Axolotl

Chapter 10 Pseudotyped Retroviruses for Infecting Axolotl

Chapter 11 Thyroxine-Induced Metamorphosis in the Axolotl (Ambystoma mexicanum).

Chapter 12 Generation of Aneurogenic Larvae by Parabiosis of Salamander Embryos

Chapter 13 In Vivo Modulation and Quantification of microRNAs During Axolotl Tail Regeneration.

Chapter 14 Derivation and Long-Term Culture of Cells from Newt Adult Limbs and Limb Blastemas.

Chapter 15 Culture and Transfection of Axolotl Cells

Chapter 16 Isolation and Culture of Neurospheres from the Adult Newt Brain

Chapter 17 Methods for Axolotl Blood Collection, Intravenous Injection, and Efficient Leukocyte Isolation from Peripheral Blood and the Regenerating Limb

Chapter 18 Assessing Cardiomyocyte Proliferative Capacity in the Newt Heart and Primary Culture

Chapter 19 Long-Term Organ Cultures of Newt Hearts

Chapter 20 In Vitro Preparation of Newt Inner Ear Sensory Epithelia as a Model for Repair and Regeneration

Chapter 21 Transgenesis in Axolotl ( Ambystoma mexicanum )

Chapter 22 Generating and Identifying Axolotls with Targeted Mutations Using Cas9 RNA-Guided Nuclease.

Chapter 23 Gene Manipulation for Regenerative Studies Using the Iberian Ribbed Newt, Pleurodeles waltl

Chapter 24 Transcriptomics using axolotls.

Chapter 25 Sal-Site: Research Resources for the Mexican Axolotl

Chapter 26 Data Mining in Newt-Omics, the Repository for Omics Data from the Newt.