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Chapter title |
Reconstitution of Clathrin Coat Disassembly for Fluorescence Microscopy and Single-Molecule Analysis
|
---|---|
Chapter number | 10 |
Book title |
Clathrin-Mediated Endoytosis
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Published in |
Methods in molecular biology, August 2018
|
DOI | 10.1007/978-1-4939-8719-1_10 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8717-7, 978-1-4939-8719-1
|
Authors |
Till Böcking, Srigokul Upadhyayula, Iris Rapoport, Benjamin R. Capraro, Tom Kirchhausen, Böcking, Till, Upadhyayula, Srigokul, Rapoport, Iris, Capraro, Benjamin R., Kirchhausen, Tom |
Abstract |
The disassembly of the clathrin lattice surrounding coated vesicles is the obligatory last step in their life cycle. It is mediated by the coordinated recruitment of auxilin and Hsc70, an ATP-driven molecular clamp. Here, we describe the preparation of reagents and the single-particle fluorescence microscopy imaging assay in which we visualize directly the Hsc70-driven uncoating of synthetic clathrin coats or clathrin-coated vesicles. |
Mendeley readers
The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 10 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 3 | 30% |
Student > Master | 2 | 20% |
Other | 1 | 10% |
Unknown | 4 | 40% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 20% |
Business, Management and Accounting | 1 | 10% |
Agricultural and Biological Sciences | 1 | 10% |
Physics and Astronomy | 1 | 10% |
Unknown | 5 | 50% |