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Clathrin-Mediated Endoytosis

Overview of attention for book
Cover of 'Clathrin-Mediated Endoytosis'

Table of Contents

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    Book Overview
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    Chapter 1 Purification of Clathrin-Coated Vesicles from Adult Rat Brain
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    Chapter 2 Preparation of Synaptosomes from Mammalian Brain by Subcellular Fractionation and Gradient Centrifugation
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    Chapter 3 Probing Endocytosis During the Cell Cycle with Minimal Experimental Perturbation
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    Chapter 4 Assaying the Contribution of Membrane Tension to Clathrin-Mediated Endocytosis
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    Chapter 5 Identifying Small-Molecule Inhibitors of the Clathrin Terminal Domain
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    Chapter 6 Acute Manipulations of Clathrin-Mediated Endocytosis at Presynaptic Nerve Terminals
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    Chapter 7 Imaging “Hot-Wired” Clathrin-Mediated Endocytosis
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    Chapter 8 Real-Time Endocytosis Measurements by Membrane Capacitance Recording at Central Nerve Terminals
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    Chapter 9 Assaying Mutants of Clathrin-Mediated Endocytosis in the Fly Eye
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    Chapter 10 Reconstitution of Clathrin Coat Disassembly for Fluorescence Microscopy and Single-Molecule Analysis
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    Chapter 11 Spatial and Temporal Aspects of Phosphoinositides in Endocytosis Studied in the Isolated Plasma Membranes
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    Chapter 12 SMrT Assay for Real-Time Visualization and Analysis of Clathrin Assembly Reactions
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    Chapter 13 Real-Time Monitoring of Clathrin Assembly Kinetics in a Reconstituted System
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    Chapter 14 Stimulated Emission Depletion (STED) Imaging of Clathrin-Mediated Endocytosis in Living Cells
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    Chapter 15 Measuring Clathrin-Coated Vesicle Formation with Single-Molecule Resolution
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    Chapter 16 Cryo-Electron Tomography of the Mammalian Synapse
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    Chapter 17 Quantitative Analysis of Clathrin-Mediated Endocytosis in Yeast by Live Cell Fluorescence Microscopy
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    Chapter 18 Using FM Dyes to Monitor Clathrin-Mediated Endocytosis in Primary Neuronal Culture
Attention for Chapter 9: Assaying Mutants of Clathrin-Mediated Endocytosis in the Fly Eye
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Chapter title
Assaying Mutants of Clathrin-Mediated Endocytosis in the Fly Eye
Chapter number 9
Book title
Clathrin-Mediated Endoytosis
Published in
Methods in molecular biology, August 2018
DOI 10.1007/978-1-4939-8719-1_9
Pubmed ID
Book ISBNs
978-1-4939-8717-7, 978-1-4939-8719-1
Authors

Elsa Lauwers, Patrik Verstreken, Lauwers, Elsa, Verstreken, Patrik

Abstract

Clathrin-mediated endocytosis plays essential roles both during and after development, and loss-of-function mutants affected in this process are mostly not viable. Different approaches have been developed to circumvent this limitation, including resorting to mosaic model organisms. We here describe the use of FLP/FRT-mediated mitotic recombination to generate Drosophila melanogaster having homozygous mutant eyes while the rest of their body is heterozygous. We then present a detailed protocol for assessing the consequences of these loss-of-function mutations on endocytosis in the photoreceptors of living fruit flies by recording electroretinograms.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 5 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2 40%
Student > Bachelor 1 20%
Researcher 1 20%
Student > Master 1 20%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 60%
Agricultural and Biological Sciences 1 20%
Neuroscience 1 20%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 August 2018.
All research outputs
#19,015,492
of 23,577,654 outputs
Outputs from Methods in molecular biology
#8,199
of 13,410 outputs
Outputs of similar age
#258,121
of 334,835 outputs
Outputs of similar age from Methods in molecular biology
#164
of 250 outputs
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So far Altmetric has tracked 13,410 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
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We're also able to compare this research output to 250 others from the same source and published within six weeks on either side of this one. This one is in the 23rd percentile – i.e., 23% of its contemporaries scored the same or lower than it.