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Clathrin-Mediated Endoytosis

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Cover of 'Clathrin-Mediated Endoytosis'

Table of Contents

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    Book Overview
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    Chapter 1 Purification of Clathrin-Coated Vesicles from Adult Rat Brain
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    Chapter 2 Preparation of Synaptosomes from Mammalian Brain by Subcellular Fractionation and Gradient Centrifugation
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    Chapter 3 Probing Endocytosis During the Cell Cycle with Minimal Experimental Perturbation
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    Chapter 4 Assaying the Contribution of Membrane Tension to Clathrin-Mediated Endocytosis
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    Chapter 5 Identifying Small-Molecule Inhibitors of the Clathrin Terminal Domain
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    Chapter 6 Acute Manipulations of Clathrin-Mediated Endocytosis at Presynaptic Nerve Terminals
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    Chapter 7 Imaging “Hot-Wired” Clathrin-Mediated Endocytosis
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    Chapter 8 Real-Time Endocytosis Measurements by Membrane Capacitance Recording at Central Nerve Terminals
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    Chapter 9 Assaying Mutants of Clathrin-Mediated Endocytosis in the Fly Eye
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    Chapter 10 Reconstitution of Clathrin Coat Disassembly for Fluorescence Microscopy and Single-Molecule Analysis
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    Chapter 11 Spatial and Temporal Aspects of Phosphoinositides in Endocytosis Studied in the Isolated Plasma Membranes
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    Chapter 12 SMrT Assay for Real-Time Visualization and Analysis of Clathrin Assembly Reactions
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    Chapter 13 Real-Time Monitoring of Clathrin Assembly Kinetics in a Reconstituted System
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    Chapter 14 Stimulated Emission Depletion (STED) Imaging of Clathrin-Mediated Endocytosis in Living Cells
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    Chapter 15 Measuring Clathrin-Coated Vesicle Formation with Single-Molecule Resolution
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    Chapter 16 Cryo-Electron Tomography of the Mammalian Synapse
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    Chapter 17 Quantitative Analysis of Clathrin-Mediated Endocytosis in Yeast by Live Cell Fluorescence Microscopy
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    Chapter 18 Using FM Dyes to Monitor Clathrin-Mediated Endocytosis in Primary Neuronal Culture
Attention for Chapter 13: Real-Time Monitoring of Clathrin Assembly Kinetics in a Reconstituted System
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Chapter title
Real-Time Monitoring of Clathrin Assembly Kinetics in a Reconstituted System
Chapter number 13
Book title
Clathrin-Mediated Endoytosis
Published in
Methods in molecular biology, August 2018
DOI 10.1007/978-1-4939-8719-1_13
Pubmed ID
Book ISBNs
978-1-4939-8717-7, 978-1-4939-8719-1
Authors

Jeffery Yong, Yan Chen, Min Wu, Yong, Jeffery, Chen, Yan, Wu, Min

Abstract

Clathrin-coated pits (ccp) are important structures that cells use for internalizing materials and regulating plasma membrane homeostasis. We had previously described an assay of reconstituting ccp assembly on sheets of basal plasma membranes. Here, we describe a workflow to adapt this system for monitoring the assembly of ccps over time using total internal reflection fluorescence (TIRF) microscopy.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 6 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 1 17%
Researcher 1 17%
Unknown 4 67%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 33%
Unknown 4 67%