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Mendeley readers
| Chapter title |
Production, Purification, and Characterization of Antibody-TNF Superfamily Ligand Fusion Proteins
|
|---|---|
| Chapter number | 18 |
| Book title |
Antibody Engineering
|
| Published in |
Methods in molecular biology, September 2018
|
| DOI | 10.1007/978-1-4939-8648-4_18 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-8647-7, 978-1-4939-8648-4
|
| Authors |
Martin Siegemund, Nadine Beha, Dafne Müller, Siegemund, Martin, Beha, Nadine, Müller, Dafne |
| Abstract |
Antibody-fusion proteins with ligands, e.g., of the TNF superfamily (TNFSF) can be adequately produced in mammalian expression systems. Here, we describe the transient production in adherent and suspension human embryonic kidney cells at laboratory scale, followed by purification procedures applying protein A and immobilized metal affinity chromatography for proteins with Fc domain and 6 × histidine-tag, respectively. In addition, characterization of the purified proteins by size exclusion chromatography is described. |
Mendeley readers
The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 9 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 3 | 33% |
| Professor > Associate Professor | 1 | 11% |
| Researcher | 1 | 11% |
| Student > Bachelor | 1 | 11% |
| Unknown | 3 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemical Engineering | 1 | 11% |
| Biochemistry, Genetics and Molecular Biology | 1 | 11% |
| Nursing and Health Professions | 1 | 11% |
| Agricultural and Biological Sciences | 1 | 11% |
| Engineering | 1 | 11% |
| Other | 0 | 0% |
| Unknown | 4 | 44% |