| Chapter title |
Phage Display and Selections on Purified Antigens
|
|---|---|
| Chapter number | 9 |
| Book title |
Antibody Engineering
|
| Published in |
Methods in molecular biology, September 2018
|
| DOI | 10.1007/978-1-4939-8648-4_9 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-8647-7, 978-1-4939-8648-4
|
| Authors |
Magali Colazet, Patrick Chames, Colazet, Magali, Chames, Patrick |
| Abstract |
The isolation of antibody fragments targeting proteins implicated in cancers and other diseases remains a crucial issue on targeted therapy or diagnostic tool development. In many case, the protein of interest, or a relevant portion of this protein such as its extracellular domain, is available as purified protein. In such cases, phage display on purified antigen is an easy and fast way to select antibody fragment able to efficiently bind this antigen. However the output of phage selection can vary significantly depending on the way to immobilize the purified antigen during selection. The following protocols describe the selection of phage antibody on purified antigen adsorbed on plastic, i.e., panning, or a selection in solution, using a biotinylated antigen as well as the corresponding screening produces, and give hints on the advantage and drawbacks of each approach. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 22 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 5 | 23% |
| Researcher | 5 | 23% |
| Student > Ph. D. Student | 4 | 18% |
| Student > Bachelor | 1 | 5% |
| Unknown | 7 | 32% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 6 | 27% |
| Agricultural and Biological Sciences | 4 | 18% |
| Arts and Humanities | 1 | 5% |
| Veterinary Science and Veterinary Medicine | 1 | 5% |
| Chemistry | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 8 | 36% |