Chapter title |
Measuring Antibody-Antigen Binding Kinetics Using Surface Plasmon Resonance
|
---|---|
Chapter number | 22 |
Book title |
Antibody Engineering
|
Published in |
Methods in molecular biology, September 2018
|
DOI | 10.1007/978-1-4939-8648-4_22 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8647-7, 978-1-4939-8648-4
|
Authors |
Stephen Hearty, Paul Leonard, Hui Ma, Richard O’Kennedy, Hearty, Stephen, Leonard, Paul, Ma, Hui, O’Kennedy, Richard |
Abstract |
Surface plasmon resonance (SPR) is now widely embraced as a technology for monitoring a diverse range of protein-protein interactions and is considered almost de rigueur for characterizing antibody-antigen interactions. The technique obviates the need to label either of the interacting species, and the binding event is visualized in real time. Thus, it is ideally suited for screening crude, unpurified antibody samples that dominate early candidate panels following antibody selection campaigns. SPR returns not only concentration and affinity data but when used correctly can resolve the discrete component kinetic parameters (association and dissociation rate constants) of the affinity interaction. Herein, we outline some SPR-based generic antibody screening configurations and methodologies in the context of expediting data-rich ranking of candidate antibody panels and ensuring that antibodies with the optimal kinetic binding characteristics are reliably identified. |
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