You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output.
Click here to find out more.
Mendeley readers
Chapter title |
Scalable In Vitro Proteasome Activity Assay
|
---|---|
Chapter number | 21 |
Book title |
The Ubiquitin Proteasome System
|
Published in |
Methods in molecular biology, September 2018
|
DOI | 10.1007/978-1-4939-8706-1_21 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8705-4, 978-1-4939-8706-1
|
Authors |
Amit Kumar Singh Gautam, Kirby Martinez-Fonts, Andreas Matouschek, Singh Gautam, Amit Kumar, Martinez-Fonts, Kirby, Matouschek, Andreas |
Abstract |
We developed a degradation assay based on fluorescent protein substrates that are efficiently recognized, unfolded, translocated, and hydrolyzed by the proteasome. The substrates consist of three components: a proteasome-binding tag, a folded domain, and an initiation region. All the components of the model substrate can be changed to modulate degradation, and the assay can be performed in parallel in 384-well plates. These properties allow the assay to be used to explore a wide range of experimental conditions and to screen proteasome modulators. |
Mendeley readers
The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 16 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 4 | 25% |
Student > Bachelor | 3 | 19% |
Student > Postgraduate | 3 | 19% |
Researcher | 2 | 13% |
Student > Doctoral Student | 1 | 6% |
Other | 0 | 0% |
Unknown | 3 | 19% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 9 | 56% |
Agricultural and Biological Sciences | 2 | 13% |
Computer Science | 1 | 6% |
Medicine and Dentistry | 1 | 6% |
Unknown | 3 | 19% |