| Chapter title |
Mutagenesis in Xenopus and Zebrafish using TALENs
|
|---|---|
| Chapter number | 16 |
| Book title |
TALENs
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-2932-0_16 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2931-3, 978-1-4939-2932-0
|
| Authors |
Yun Liu, Hui Zhao, Christopher H. K. Cheng, Liu, Yun, Zhao, Hui, Cheng, Christopher H. K. |
| Abstract |
Transcription activator-like effector nucleases (TALENs) have been proven to be effective for gene specific targeting across species. Here we describe the validated protocol for TALEN assembly as well as methods for generating gene knockout animals of Xenopus and zebrafish. This protocol covers selection of TALEN targeting sites, TALEN assembly with a modified Golden Gate method, injection of TALEN mRNAs into Xenopus and zebrafish embryos as well as the detection of somatic and germ-line transmitted mutations. Finally, the establishment of knockout Xenopus and zebrafish lines is also described. This protocol will facilitate broader applications of TALENs in developmental biology. |
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Geographical breakdown
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|---|---|---|
| Unknown | 4 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Professor | 1 | 25% |
| Librarian | 1 | 25% |
| Student > Ph. D. Student | 1 | 25% |
| Student > Postgraduate | 1 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 1 | 25% |
| Agricultural and Biological Sciences | 1 | 25% |
| Neuroscience | 1 | 25% |
| Medicine and Dentistry | 1 | 25% |