| Chapter title |
Genome Editing in Rats Using TALE Nucleases
|
|---|---|
| Chapter number | 18 |
| Book title |
TALENs
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-2932-0_18 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2931-3, 978-1-4939-2932-0
|
| Authors |
Laurent Tesson, Séverine Remy, Séverine Ménoret, Claire Usal, Reynald Thinard, Chloé Savignard, Anne De Cian, Carine Giovannangeli, Jean-Paul Concordet, Ignacio Anegon, Tesson, Laurent, Remy, Séverine, Ménoret, Séverine, Usal, Claire, Thinard, Reynald, Savignard, Chloé, Cian, Anne De, Giovannangeli, Carine, Concordet, Jean-Paul, Anegon, Ignacio |
| Abstract |
The rat is an important animal model to understand gene function and model human diseases. Since recent years, the development of gene-specific nucleases has become important for generating new rat models of human diseases, to analyze the role of genes and to generate human antibodies. Transcription activator-like (TALE) nucleases efficiently create gene-specific knockout rats and lead to the possibility of gene targeting by homology-directed recombination (HDR) and generating knock-in rats. We describe a detailed protocol for generating knockout and knock-in rats via microinjection of TALE nucleases into fertilized eggs. This technology is an efficient, cost- and time-effective method for creating new rat models. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 12 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 4 | 33% |
| Student > Ph. D. Student | 2 | 17% |
| Lecturer | 1 | 8% |
| Student > Master | 1 | 8% |
| Professor > Associate Professor | 1 | 8% |
| Other | 0 | 0% |
| Unknown | 3 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 5 | 42% |
| Agricultural and Biological Sciences | 4 | 33% |
| Unknown | 3 | 25% |