| Chapter title |
GeneKnockout by Targeted Mutagenesis in a Hemimetabolous Insect, the Two-Spotted Cricket Gryllus bimaculatus , using TALENs
|
|---|---|
| Chapter number | 12 |
| Book title |
TALENs
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-2932-0_12 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2931-3, 978-1-4939-2932-0
|
| Authors |
Takahito Watanabe, Sumihare Noji, Taro Mito, Watanabe, Takahito, Noji, Sumihare, Mito, Taro |
| Abstract |
Hemimetabolous, or incompletely metamorphosing, insects are phylogenetically basal. These insects include many deleterious species. The cricket, Gryllus bimaculatus, is an emerging model for hemimetabolous insects, based on the success of RNA interference (RNAi)-based gene-functional analyses and transgenic technology. Taking advantage of genome-editing technologies in this species would greatly promote functional genomics studies. Genome editing using transcription activator-like effector nucleases (TALENs) has proven to be an effective method for site-specific genome manipulation in various species. TALENs are artificial nucleases that are capable of inducing DNA double-strand breaks into specified target sequences. Here, we describe a protocol for TALEN-based gene knockout in G. bimaculatus, including a mutant selection scheme via mutation detection assays, for generating homozygous knockout organisms. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 22 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 7 | 32% |
| Student > Master | 5 | 23% |
| Other | 4 | 18% |
| Student > Doctoral Student | 2 | 9% |
| Professor > Associate Professor | 2 | 9% |
| Other | 2 | 9% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 13 | 59% |
| Biochemistry, Genetics and Molecular Biology | 4 | 18% |
| Environmental Science | 2 | 9% |
| Neuroscience | 2 | 9% |
| Unknown | 1 | 5% |