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Gene Synthesis

Overview of attention for book
Cover of 'Gene Synthesis'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Building block synthesis using the polymerase chain assembly method.
  3. Altmetric Badge
    Chapter 2 Oligonucleotide assembly in yeast to produce synthetic DNA fragments.
  4. Altmetric Badge
    Chapter 3 TopDown Real-Time Gene Synthesis
  5. Altmetric Badge
    Chapter 4 De Novo DNA Synthesis Using Single-Molecule PCR
  6. Altmetric Badge
    Chapter 5 SLIC: A Method for Sequence- and Ligation-Independent Cloning.
  7. Altmetric Badge
    Chapter 6 Assembly of Standardized DNA Parts Using BioBrick Ends in E. coli.
  8. Altmetric Badge
    Chapter 7 Assembling DNA Fragments by USER Fusion
  9. Altmetric Badge
    Chapter 8 Fusion PCR via Novel Overlap Sequences
  10. Altmetric Badge
    Chapter 9 Using recombineering to generate point mutations: the oligonucleotide-based "hit and fix" method.
  11. Altmetric Badge
    Chapter 10 Using Recombineering to Generate Point Mutations:galK-Based Positive-Negative Selection Method.
  12. Altmetric Badge
    Chapter 11 Assembling Large DNA Segments in Yeast
  13. Altmetric Badge
    Chapter 12 Recursive construction of perfect DNA molecules and libraries from imperfect oligonucleotides.
  14. Altmetric Badge
    Chapter 13 Cloning whole bacterial genomes in yeast.
  15. Altmetric Badge
    Chapter 14 Production of infectious poliovirus from synthetic viral genomes.
  16. Altmetric Badge
    Chapter 15 In silico design of functional DNA constructs.
  17. Altmetric Badge
    Chapter 16 Using DNAWorks in Designing Oligonucleotides for PCR-Based Gene Synthesis
  18. Altmetric Badge
    Chapter 17 De Novo Gene Synthesis Design Using TmPrime Software
  19. Altmetric Badge
    Chapter 18 Design-A-Gene with GeneDesign.
  20. Altmetric Badge
    Chapter 19 Leading a Successful iGEM Team.
  21. Altmetric Badge
    Chapter 20 The Build-a-Genome Course.
  22. Altmetric Badge
    Chapter 21 DNA Synthesis Security
Attention for Chapter 13: Cloning whole bacterial genomes in yeast.
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Chapter title
Cloning whole bacterial genomes in yeast.
Chapter number 13
Book title
Gene Synthesis
Published in
Methods in molecular biology, February 2012
DOI 10.1007/978-1-61779-564-0_13
Pubmed ID
Book ISBNs
978-1-61779-563-3, 978-1-61779-564-0
Authors

Benders GA, Gwynedd A. Benders, Benders, Gwynedd A.

Abstract

Many bacterial and archaeal genomes are of a similar size to molecules that have been cloned in the yeast Saccharomyces cerevisiae and thus might be clonable as single, circular episomes in this host. Yeast offers a variety of efficient tools for the manipulation and study of cloned DNA. One strategy to clone a genome in yeast is to cotransform yeast spheroplasts with the genome of interest and a linear yeast vector whose termini are homologous to a spot in the genome. Clones are selected on auxotrophic medium and then screened for completeness and size; they may also be sequenced.

X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 48 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 2%
Unknown 47 98%

Demographic breakdown

Readers by professional status Count As %
Researcher 9 19%
Student > Ph. D. Student 8 17%
Student > Master 6 13%
Professor > Associate Professor 5 10%
Other 4 8%
Other 7 15%
Unknown 9 19%
Readers by discipline Count As %
Agricultural and Biological Sciences 23 48%
Biochemistry, Genetics and Molecular Biology 9 19%
Chemistry 2 4%
Computer Science 1 2%
Immunology and Microbiology 1 2%
Other 1 2%
Unknown 11 23%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 June 2012.
All research outputs
#15,245,883
of 22,668,244 outputs
Outputs from Methods in molecular biology
#5,287
of 13,025 outputs
Outputs of similar age
#165,517
of 250,724 outputs
Outputs of similar age from Methods in molecular biology
#258
of 461 outputs
Altmetric has tracked 22,668,244 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,025 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 45th percentile – i.e., 45% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 250,724 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 22nd percentile – i.e., 22% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 461 others from the same source and published within six weeks on either side of this one. This one is in the 34th percentile – i.e., 34% of its contemporaries scored the same or lower than it.