Chapter title |
In Vivo Fluorescence Reflectance Imaging with Subcutaneous Mouse Tumor Models.
|
---|---|
Chapter number | 3 |
Book title |
In Vivo Fluorescence Imaging
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3721-9_3 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3719-6, 978-1-4939-3721-9
|
Authors |
Jie Cao, Mingzhou Zhou |
Editors |
Mingfeng Bai |
Abstract |
Optical imaging is undoubtedly one of the most versatile and widely used imaging techniques in both research and clinical practice. Among optical imaging technologies, fluorescence imaging is the most popularly used and has become an essential tool in biomedical science. A key component of fluorescence imaging is fluorescence-producing reporters, including fluorescent dyes and conjugates, as well as fluorescent proteins. For in vivo imaging applications, fluorophores with long emission at the near-infrared (NIR) region are generally preferred to overcome the photon attenuation in living tissue. Here, we describe the in vivo fluorescence imaging of an integrin αυβ3 targeted NIR fluorescent probe (cRGD-ICG-Der-02) using subcutaneous mouse tumor models. |
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