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In Vivo Fluorescence Imaging

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Cover of 'In Vivo Fluorescence Imaging'

Table of Contents

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    Book Overview
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    Chapter 1 Before In Vivo Imaging: Evaluation of Fluorescent Probes Using Fluorescence Microscopy, Multiplate Reader, and Cytotoxicity Assays.
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    Chapter 2 Bioconjugation Methods for Coupling Targeting Ligands with Fluorescent Dyes.
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    Chapter 3 In Vivo Fluorescence Reflectance Imaging with Subcutaneous Mouse Tumor Models.
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    Chapter 4 FRET Imaging of Enzymatic Activities Using Smart Probes.
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    Chapter 5 Fluorescence Imaging of Inflammation in Live Animals.
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    Chapter 6 Fluorescence Lifetime Imaging of Cancer In Vivo.
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    Chapter 7 Noninvasive Imaging of Fluorescent Reporters in Small Rodent Models Using Fluorescence Molecular Tomography.
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    Chapter 8 In Vivo Tumor Angiogenesis Imaging Using Peptide-Based Near-Infrared Fluorescent Probes.
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    Chapter 9 Intraoperative Fluorescence Imaging and Multimodal Surgical Navigation Using Goggle System.
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    Chapter 10 Utilization of Near-Infrared Fluorescent Imaging for Pharmaceutically Relevant Applications.
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    Chapter 11 In Vivo Cell Tracking Using Two-Photon Microscopy.
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    Chapter 12 Small-Animal Imaging Using Diffuse Fluorescence Tomography.
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    Chapter 13 In Vivo Metal Ion Imaging Using Fluorescent Sensors.
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    Chapter 14 In Vivo Fluorescence Imaging
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    Chapter 15 In Vivo Fluorescence Imaging in the Second Near-Infrared Window Using Carbon Nanotubes.
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    Chapter 16 Ex Vivo Imaging, Biodistribution, and Histological Study in Addition to In Vivo Imaging.
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    Chapter 17 Experimental Design and Data Analysis of In Vivo Fluorescence Imaging Studies.
Attention for Chapter 4: FRET Imaging of Enzymatic Activities Using Smart Probes.
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Chapter title
FRET Imaging of Enzymatic Activities Using Smart Probes.
Chapter number 4
Book title
In Vivo Fluorescence Imaging
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3721-9_4
Pubmed ID
Book ISBNs
978-1-4939-3719-6, 978-1-4939-3721-9
Authors

Jinbo Li, Yan Zhang, Zhen Cheng

Editors

Mingfeng Bai

Abstract

Tumor-related enzymes are extensively involved in the occurrence, development, invasion, and metastasis of tumors, indicating they hold the potential to serve as biomarkers for tumor diagnosis and treatment. Smart probes based on characteristic activities of these enzymes and fluorescence resonance energy transfer (FRET) have been widely developed for fluorescent imaging of enzymatic activities. Here, we describe the detailed chemical strategies for construction of smart probe and its application for FRET imaging of fibroblast activation protein alpha in vitro and in vivo.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 14 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 14 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 29%
Student > Doctoral Student 2 14%
Professor 1 7%
Student > Ph. D. Student 1 7%
Professor > Associate Professor 1 7%
Other 0 0%
Unknown 5 36%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 21%
Chemistry 3 21%
Medicine and Dentistry 1 7%
Unknown 7 50%