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Mendeley readers
| Chapter title |
Bioconjugation Methods for Coupling Targeting Ligands with Fluorescent Dyes.
|
|---|---|
| Chapter number | 2 |
| Book title |
In Vivo Fluorescence Imaging
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3721-9_2 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3719-6, 978-1-4939-3721-9
|
| Authors |
Xiaoxi Ling |
| Editors |
Mingfeng Bai |
| Abstract |
Targeted molecular imaging probes are essential tools for visualization of specific molecular processes in cells and living systems. Among these, targeted fluorescent probes are widely used due to the high sensitivity and resolution of fluorescence imaging. The conventional strategy for developing targeted fluorescent probes is to couple targeting ligands with fluorescent dyes by covalent bond via bioconjugation. Here, we describe several commonly used bioconjugation methods, from traditional amide and thiol coupling, to metal-catalyzed coupling reaction and catalyst free cycloaddition. |
Mendeley readers
The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 12 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 1 | 8% |
| Student > Doctoral Student | 1 | 8% |
| Unknown | 10 | 83% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 1 | 8% |
| Chemistry | 1 | 8% |
| Unknown | 10 | 83% |