| Chapter title |
Plant Pathology
|
|---|---|
| Chapter number | 14 |
| Book title |
Plant Pathology
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2620-6_14 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2619-0, 978-1-4939-2620-6
|
| Authors |
Chikh-Ali, Mohamad, Karasev, Alexander V, Mohamad Chikh-Ali, Alexander V. Karasev, Karasev, Alexander V. |
| Abstract |
Immunocapture-reverse transcription-polymerase chain reaction (IC-RT-PCR) is a sensitive, reproducible, and robust method for the detection and identification of RNA viruses. The IC step provides a simple method to isolate virus particles from plant tissue, particularly when inhibitory substances are present, and thus enables subsequent use of RT-PCR amplification for large-scale virus testing and typing. The multiplex format of the PCR is often used for the detection and identification of multiple virus/strain simultaneously to save time, labor, and cost. Potato virus Y (PVY) is one of the most economically important viruses infecting potato worldwide. PVY exists as a complex of at least nine strains and many more unclassified recombinants that vary in their genome structures, phenotypes, and their economic importance. In the current chapter, a detailed protocol of an IC-based, multiplex RT-PCR assay for the detection and identification of various PVY strains is described. |
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