Chapter title |
Metagenomic next-generation sequencing of viruses infecting grapevines.
|
---|---|
Chapter number | 23 |
Book title |
Plant Pathology
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2620-6_23 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2619-0, 978-1-4939-2620-6
|
Authors |
Burger, Johan T, Maree, Hans J, Johan T. Burger, Hans J. Maree |
Abstract |
Next-generation sequencing (NGS) technologies, for the first time, provide a truly "complete" representation of the viral (and other) pathogens present in a host organism. This is achieved in an unbiased way, and without any prior biological or molecular knowledge of these pathogen(s). During recent years a number of broad approaches, for most of the popular NGS platforms, have been developed. Here we describe such a protocol-one that accurately and reliably analyze viruses (and viroids) infecting grapevine. Our strategy relies on the synthesis of cDNA sequencing libraries from dsRNA, extracted from diseased grapevine tissues; the sequencing of these on an Illumina platform, and a streamlined bioinformatics pipeline to analyze the NGS data, yielding the virus composition (virome) of a specific grapevine tissue type, organ, entire plant, or even a vineyard. |
X Demographics
Geographical breakdown
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
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Brazil | 2 | 4% |
Switzerland | 1 | 2% |
Unknown | 43 | 93% |
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Researcher | 11 | 24% |
Student > Master | 7 | 15% |
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Student > Doctoral Student | 3 | 7% |
Other | 7 | 15% |
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Immunology and Microbiology | 2 | 4% |
Environmental Science | 1 | 2% |
Unspecified | 1 | 2% |
Other | 2 | 4% |
Unknown | 9 | 20% |