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Peptide Microarrays

Overview of attention for book
Cover of 'Peptide Microarrays'

Table of Contents

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    Book Overview
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    Chapter 1 Peptide Arrays on Planar Supports.
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    Chapter 2 High-Throughput Microarray Incubations Using Multi-Well Chambers.
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    Chapter 3 Analysis of Protein Tyrosine Kinase Specificity Using Positional Scanning Peptide Microarrays.
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    Chapter 4 Secondary Structure Determination of Peptides and Proteins After Immobilization.
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    Chapter 5 Peptides and Anti-peptide Antibodies for Small and Medium Scale Peptide and Anti-peptide Affinity Microarrays: Antigenic Peptide Selection, Immobilization, and Processing.
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    Chapter 6 Low-Cost Peptide Microarrays for Mapping Continuous Antibody Epitopes
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    Chapter 7 The Peptide Microarray-Based Resonance Light Scattering Assay for Sensitively Detecting Intracellular Kinase Activity.
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    Chapter 8 Anomalous Reflection of Gold: A Novel Platform for Biochips.
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    Chapter 9 High-Throughput Peptide Screening on a Bimodal Imprinting Chip Through MS-SPRi Integration.
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    Chapter 10 Analyzing Peptide Microarray Data with the R pepStat Package.
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    Chapter 11 Chemoselective Strategies to Peptide and Protein Bioprobes Immobilization on Microarray Surfaces.
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    Chapter 12 Manufacturing of Peptide Microarrays Based on Catalyst-Free Click Chemistry.
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    Chapter 13 Clickable Polymeric Coating for Oriented Peptide Immobilization.
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    Chapter 14 Oriented Peptide Immobilization on Microspheres.
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    Chapter 15 A Cell Microarray Format: A Peptide Release System Using a Photo-Cleavable Linker for Cell Toxicity and Cell Uptake Analysis.
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    Chapter 16 Peptide Microarrays for Medical Applications in Autoimmunity, Infection, and Cancer.
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    Chapter 17 Synthetic Peptide-Based ELISA and ELISpot Assay for Identifying Autoantibody Epitopes.
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    Chapter 18 IgE and IgG4 Epitope Mapping of Food Allergens with a Peptide Microarray Immunoassay.
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    Chapter 19 IgE Epitope Mapping Using Peptide Microarray Immunoassay.
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    Chapter 20 Spot Synthesis: An Optimized Microarray to Detect IgE Epitopes.
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    Chapter 21 Mapping of Epitopes Occurring in Bovine αs1-Casein Variants by Peptide Microarray Immunoassay.
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    Chapter 22 Erratum To: Peptide Arrays on Planar Supports.
Attention for Chapter 5: Peptides and Anti-peptide Antibodies for Small and Medium Scale Peptide and Anti-peptide Affinity Microarrays: Antigenic Peptide Selection, Immobilization, and Processing.
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Chapter title
Peptides and Anti-peptide Antibodies for Small and Medium Scale Peptide and Anti-peptide Affinity Microarrays: Antigenic Peptide Selection, Immobilization, and Processing.
Chapter number 5
Book title
Peptide Microarrays
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3037-1_5
Pubmed ID
26490467
Book ISBNs
978-1-4939-3036-4, 978-1-4939-3037-1
Authors

Fan Zhang, Andrea Briones, Mikhail Soloviev

Editors

Marina Cretich, Marcella Chiari

Abstract

This chapter describes the principles of selection of antigenic peptides for the development of anti-peptide antibodies for use in microarray-based multiplex affinity assays and also with mass-spectrometry detection. The methods described here are mostly applicable to small to medium scale arrays. Although the same principles of peptide selection would be suitable for larger scale arrays (with 100+ features) the actual informatics software and printing methods may well be different. Because of the sheer number of proteins/peptides to be processed and analyzed dedicated software capable of processing all the proteins and an enterprise level array robotics may be necessary for larger scale efforts. This report aims to provide practical advice to those who develop or use arrays with up to ~100 different peptide or protein features.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 7 100%

Demographic breakdown

Readers by professional status Count As %
Other 2 29%
Student > Bachelor 1 14%
Researcher 1 14%
Student > Master 1 14%
Unknown 2 29%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 43%
Pharmacology, Toxicology and Pharmaceutical Science 1 14%
Economics, Econometrics and Finance 1 14%
Unknown 2 29%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 August 2016.
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#18,467,278
of 22,882,389 outputs
Outputs from Methods in molecular biology
#7,923
of 13,131 outputs
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#284,557
of 393,703 outputs
Outputs of similar age from Methods in molecular biology
#845
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