| Chapter title |
Spot Synthesis: An Optimized Microarray to Detect IgE Epitopes.
|
|---|---|
| Chapter number | 20 |
| Book title |
Peptide Microarrays
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3037-1_20 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3036-4, 978-1-4939-3037-1
|
| Authors |
Salvatore G. De-Simone, Paloma Napoleão-Pêgo, Thatiane S. De-Simone |
| Editors |
Marina Cretich, Marcella Chiari |
| Abstract |
Peptide microarrays have become increasingly more affordable in recent years with the SPOT technique being one of the most frequently used methods for synthesis and screening of peptides in arrays. Here, a protocol is presented for the identification of the amino acid sites involved in the conversion of human IgG to IgE response during the passive administration of therapeutic, anti-snake venom sera. Similarly, the minimal region of both the IgG and IgE binding epitopes, important for its interaction with ligand, were identified. As the ratio of concentrations for IgG to IgE in human serum is 1:10,000, also presented is a reproductive protocol of chemiluminescence-scanning for the detection of both immunoglobulins. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Brazil | 1 | 5% |
| Unknown | 20 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 6 | 29% |
| Student > Bachelor | 3 | 14% |
| Professor > Associate Professor | 2 | 10% |
| Student > Doctoral Student | 1 | 5% |
| Other | 1 | 5% |
| Other | 4 | 19% |
| Unknown | 4 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 6 | 29% |
| Agricultural and Biological Sciences | 4 | 19% |
| Unspecified | 1 | 5% |
| Computer Science | 1 | 5% |
| Chemistry | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 7 | 33% |