Chapter title |
High-Throughput Microarray Incubations Using Multi-Well Chambers.
|
---|---|
Chapter number | 2 |
Book title |
Peptide Microarrays
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3037-1_2 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3036-4, 978-1-4939-3037-1
|
Authors |
Zerweck, Johannes, Reimer, Ulf, Jansong, Janina, Pawlowski, Nikolaus, Tersch, Christoph, Eckey, Maren, Knaute, Tobias, Johannes Zerweck, Ulf Reimer, Janina Jansong, Nikolaus Pawlowski, Christoph Tersch, Maren Eckey, Tobias Knaute |
Editors |
Marina Cretich, Marcella Chiari |
Abstract |
Peptide microarrays are ideal tools for a variety of applications ranging from epitope mapping to immune monitoring. Here we present a method for high-throughput screening of biological samples using only standard microtiter plate equipment. Parallel incubation of a large number of samples with a small library of peptides is enabled by printing multiple identical mini-arrays on one microarray slide and further combining four slides to yield an incubation frame possessing the dimensions of a 96-well microtiter plate. Applying conventional lab equipment such as ELISA washers, hundreds of samples can be processed in 1 day yielding approx. 200 data points in triplicates per sample. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 1 | 100% |
Readers by discipline | Count | As % |
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Immunology and Microbiology | 1 | 100% |