| Chapter title |
SUMO
|
|---|---|
| Chapter number | 15 |
| Book title |
SUMO
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-6358-4_15 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6356-0, 978-1-4939-6358-4
|
| Authors |
Bawa-Khalfe, Tasneem, Tasneem Bawa-Khalfe |
| Editors |
Manuel S. Rodriguez |
| Abstract |
SUMO posttranslational modification directs gene transcription and epigenetic programming to support normal cell function. The dynamic nature of SUMO-modification makes it difficult to identify endogenous protein substrates. Isolation of chromatin-bound SUMO targets is exceptionally challenging, as conventional immunoprecipitation assays are inefficient at concentrating this protein population. This chapter describes a protocol that effectively precipitates chromatin-associated fractions of SUMOylated heterochromatin protein 1α in cultured cells. Techniques to enrich endogenous SUMO substrates at the chromatin are also demonstrated and discussed. This approach could be adapted to evaluate chromatin-bound SUMO targets in additional in vivo systems. |
X Demographics
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|---|---|---|
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|---|---|---|
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Mendeley readers
Geographical breakdown
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|---|---|---|
| Unknown | 7 | 100% |
Demographic breakdown
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|---|---|---|
| Researcher | 2 | 29% |
| Student > Ph. D. Student | 1 | 14% |
| Lecturer > Senior Lecturer | 1 | 14% |
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| Readers by discipline | Count | As % |
|---|---|---|
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| Unknown | 3 | 43% |