Chapter title |
SUMO
|
---|---|
Chapter number | 9 |
Book title |
SUMO
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-6358-4_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6356-0, 978-1-4939-6358-4
|
Authors |
Eckhoff, Julia, Dohmen, R Jürgen, Julia Eckhoff, R. Jürgen Dohmen |
Editors |
Manuel S. Rodriguez |
Abstract |
SUMO-specific proteases, known as Ulps in baker's yeast and SENPs in humans, have important roles in controlling the dynamics of SUMO-modified proteins. They display distinct modes of action and specificity, in that they may act on the SUMO precursor, mono-sumoylated, and/or polysumoylated proteins, and they might be specific for substrates with certain SUMO paralogs. SUMO chains may be dismantled either by endo or exo mechanisms. Biochemical characterization of a protease usually requires purification of the protein of interest. Developing a purification protocol, however, can be very difficult, and in some cases, isolation of a protease in its pure form may go along with a substantial loss of activity. To characterize the reaction mechanism of Ulps, we have developed an in vitro assay, which makes use of substrates endowed with artificial poly-SUMO chains of defined lengths, and S. cerevisiae Ulp enzymes in crude extract from E. coli. This fast and economic approach should be applicable to SUMO-specific proteases from other species as well. |
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