Chapter title |
Analysis of the Involuting Mouse Mammary Gland: An In Vivo Model for Cell Death.
|
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Chapter number | 7 |
Book title |
Mammary Gland Development
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6475-8_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6473-4, 978-1-4939-6475-8
|
Authors |
Bethan Lloyd-Lewis, Timothy J. Sargeant, Peter A. Kreuzaler, Henrike K. Resemann, Sara Pensa, Christine J. Watson, Lloyd-Lewis, Bethan, Sargeant, Timothy J, Kreuzaler, Peter A, Resemann, Henrike K, Pensa, Sara, Watson, Christine J, Sargeant, Timothy J., Kreuzaler, Peter A., Resemann, Henrike K., Watson, Christine J. |
Editors |
Finian Martin, Torsten Stein, Jillian Howlin |
Abstract |
Involution of the mammary gland occurs at the end of every period of lactation and is an essential process to return the gland to a pre-pregnant state in readiness for the next pregnancy. Involution is a complex process of regulated alveolar cell death coupled with tissue remodeling and requires exquisite control of transcription and signaling. These processes can be investigated using a variety of molecular and morphological approaches.In this chapter we describe how to initiate involution and collect mammary glands, measure involution morphologically, and quantify lysosomal leakiness in mammary tissue and in cultured mammary epithelial cells. These procedures encompass a range of microscopy and molecular biology techniques. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 15 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 4 | 27% |
Student > Bachelor | 3 | 20% |
Professor | 2 | 13% |
Student > Ph. D. Student | 2 | 13% |
Student > Doctoral Student | 1 | 7% |
Other | 2 | 13% |
Unknown | 1 | 7% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 8 | 53% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 13% |
Nursing and Health Professions | 1 | 7% |
Agricultural and Biological Sciences | 1 | 7% |
Medicine and Dentistry | 1 | 7% |
Other | 0 | 0% |
Unknown | 2 | 13% |