Mammary Gland Development
Springer New York
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Timeline
Mendeley readers
| Chapter title |
Contractility Assay for Established Myoepithelial Cell Lines.
|
|---|---|
| Chapter number | 8 |
| Book title |
Mammary Gland Development
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6475-8_8 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6473-4, 978-1-4939-6475-8
|
| Authors |
Stéphanie Cagnet, Marina A. Glukhova, Karine Raymond |
| Editors |
Finian Martin, Torsten Stein, Jillian Howlin |
| Abstract |
The capacity of mammary myoepithelial cells to contract in response to suckling stimuli is essential for lactation. We describe here a protocol for studying the contractile activity of myoepithelial cells in vitro. This protocol includes the establishment of stable myoepithelial cell lines from mouse mammary glands and quantitative evaluation of the contraction and subsequent relaxation of cultured myoepithelial cells in response to oxytocin. It can be used for analyses of mouse mutants with gene deletions or overexpression altering myoepithelial cell function. |
Mendeley readers
The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 4 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 1 | 25% |
| Unknown | 3 | 75% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 1 | 25% |
| Unknown | 3 | 75% |