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Campylobacter jejuni

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Cover of 'Campylobacter jejuni'

Table of Contents

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    Book Overview
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    Chapter 1 Campylobacter jejuni Isolation/Enumeration from Environmental Samples.
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    Chapter 2 Recovery of Campylobacter spp. from Food and Environmental Sources.
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    Chapter 3 Methods for Isolation, Purification, and Propagation of Bacteriophages of Campylobacter jejuni.
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    Chapter 4 Methods to Study Antimicrobial Resistance in Campylobacter jejuni.
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    Chapter 5 Method of Peptide Nucleic Acid (PNA)-Mediated Antisense Inhibition of Gene Expression in Campylobacter jejuni.
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    Chapter 6 Identification of Ligand-Receptor Interactions: Ligand Molecular Arrays, SPR and NMR Methodologies.
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    Chapter 7 Characterization of High Affinity Iron Acquisition Systems in Campylobacter jejuni.
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    Chapter 8 Method for the Successful Crystallization of the Ferric Uptake Regulator from Campylobacter jejuni.
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    Chapter 9 Methods for Initial Characterization of Campylobacter jejuni Bacteriophages.
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    Chapter 10 Methods to Assess the Direct Interaction of C. jejuni with Mucins.
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    Chapter 11 Methods to Study Campylobacter jejuni Adherence to and Invasion of Host Epithelial Cells.
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    Chapter 12 Assays to Study the Interaction of Campylobacter jejuni with the Mucosal Surface.
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    Chapter 13 Characterization of Ligand-Receptor Interactions: Chemotaxis, Biofilm, Cell Culture Assays, and Animal Model Methodologies.
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    Chapter 14 Using Galleria mellonella as an Infection Model for Campylobacter jejuni Pathogenesis.
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    Chapter 15 Mouse Models for Campylobacter jejuni Colonization and Infection.
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    Chapter 16 Metabolomic Analysis of Campylobacter jejuni by Direct-Injection Electrospray Ionization Mass Spectrometry.
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    Chapter 17 Methods for Genome-Wide Methylome Profiling of Campylobacter jejuni.
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    Chapter 18 Characterizing Glycoproteins by Mass Spectrometry in Campylobacter jejuni.
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    Chapter 19 Methods for Proteome Analysis of Campylobacter jejuni Using 2-D Electrophoresis.
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    Chapter 20 Analyzing Prokaryotic RNA-Seq Data: A Case Study Identifying Holo-Fur Regulated Genes in Campylobacter jejuni.
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    Chapter 21 Generation and Screening of an Insertion Sequencing-Compatible Mutant Library of Campylobacter jejuni.
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    Chapter 22 Erratum to: Assays to Study the Interaction of Campylobacter jejuni with the Mucosal Surface
Attention for Chapter 2: Recovery of Campylobacter spp. from Food and Environmental Sources.
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Chapter title
Recovery of Campylobacter spp. from Food and Environmental Sources.
Chapter number 2
Book title
Campylobacter jejuni
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6536-6_2
Pubmed ID
Book ISBNs
978-1-4939-6534-2, 978-1-4939-6536-6
Authors

Catherine D. Carrillo, Robyn Kenwell, Irene Iugovaz, Omar A. Oyarzabal

Editors

James Butcher, Alain Stintzi

Abstract

The recovery of Campylobacter species from food and environmental sources is challenging due to the slow growth of these bacteria and the need to suppress competing organisms during the isolation procedures. The addition of multiple selective antimicrobials to growth media can negatively impact recovery of some Campylobacter spp. Here, we describe our current method for the isolation of thermotolerant Campylobacter species, mainly C. jejuni and C. coli, from food and environmental samples. We emphasize the use of membrane filtration during plating for the specific isolation of Campylobacter spp. and a reduced use of antimicrobial supplements throughout the whole isolation process.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 20 100%

Demographic breakdown

Readers by professional status Count As %
Student > Postgraduate 2 10%
Researcher 2 10%
Student > Ph. D. Student 2 10%
Other 1 5%
Lecturer 1 5%
Other 3 15%
Unknown 9 45%
Readers by discipline Count As %
Environmental Science 2 10%
Biochemistry, Genetics and Molecular Biology 2 10%
Immunology and Microbiology 2 10%
Medicine and Dentistry 2 10%
Agricultural and Biological Sciences 1 5%
Other 2 10%
Unknown 9 45%