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Campylobacter jejuni

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Cover of 'Campylobacter jejuni'

Table of Contents

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    Book Overview
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    Chapter 1 Campylobacter jejuni Isolation/Enumeration from Environmental Samples.
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    Chapter 2 Recovery of Campylobacter spp. from Food and Environmental Sources.
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    Chapter 3 Methods for Isolation, Purification, and Propagation of Bacteriophages of Campylobacter jejuni.
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    Chapter 4 Methods to Study Antimicrobial Resistance in Campylobacter jejuni.
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    Chapter 5 Method of Peptide Nucleic Acid (PNA)-Mediated Antisense Inhibition of Gene Expression in Campylobacter jejuni.
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    Chapter 6 Identification of Ligand-Receptor Interactions: Ligand Molecular Arrays, SPR and NMR Methodologies.
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    Chapter 7 Characterization of High Affinity Iron Acquisition Systems in Campylobacter jejuni.
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    Chapter 8 Method for the Successful Crystallization of the Ferric Uptake Regulator from Campylobacter jejuni.
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    Chapter 9 Methods for Initial Characterization of Campylobacter jejuni Bacteriophages.
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    Chapter 10 Methods to Assess the Direct Interaction of C. jejuni with Mucins.
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    Chapter 11 Methods to Study Campylobacter jejuni Adherence to and Invasion of Host Epithelial Cells.
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    Chapter 12 Assays to Study the Interaction of Campylobacter jejuni with the Mucosal Surface.
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    Chapter 13 Characterization of Ligand-Receptor Interactions: Chemotaxis, Biofilm, Cell Culture Assays, and Animal Model Methodologies.
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    Chapter 14 Using Galleria mellonella as an Infection Model for Campylobacter jejuni Pathogenesis.
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    Chapter 15 Mouse Models for Campylobacter jejuni Colonization and Infection.
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    Chapter 16 Metabolomic Analysis of Campylobacter jejuni by Direct-Injection Electrospray Ionization Mass Spectrometry.
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    Chapter 17 Methods for Genome-Wide Methylome Profiling of Campylobacter jejuni.
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    Chapter 18 Characterizing Glycoproteins by Mass Spectrometry in Campylobacter jejuni.
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    Chapter 19 Methods for Proteome Analysis of Campylobacter jejuni Using 2-D Electrophoresis.
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    Chapter 20 Analyzing Prokaryotic RNA-Seq Data: A Case Study Identifying Holo-Fur Regulated Genes in Campylobacter jejuni.
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    Chapter 21 Generation and Screening of an Insertion Sequencing-Compatible Mutant Library of Campylobacter jejuni.
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    Chapter 22 Erratum to: Assays to Study the Interaction of Campylobacter jejuni with the Mucosal Surface
Attention for Chapter 19: Methods for Proteome Analysis of Campylobacter jejuni Using 2-D Electrophoresis.
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Chapter title
Methods for Proteome Analysis of Campylobacter jejuni Using 2-D Electrophoresis.
Chapter number 19
Book title
Campylobacter jejuni
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6536-6_19
Pubmed ID
Book ISBNs
978-1-4939-6534-2, 978-1-4939-6536-6
Authors

Ramila C. Rodrigues, Nabila Haddad, Odile Tresse

Editors

James Butcher, Alain Stintzi

Abstract

This chapter describes protocols used for two-dimensional electrophoretic analysis of the proteome or subproteome of Campylobacter jejuni, a major human food-borne pathogen. The following protocols, adapted to Campylobacter strains, include all the steps from cultivation to gel-support protein separation.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 4 100%

Demographic breakdown

Readers by professional status Count As %
Unspecified 1 25%
Professor > Associate Professor 1 25%
Researcher 1 25%
Unknown 1 25%
Readers by discipline Count As %
Veterinary Science and Veterinary Medicine 1 25%
Unspecified 1 25%
Immunology and Microbiology 1 25%
Unknown 1 25%