| Chapter title |
Assays to Study the Interaction of Campylobacter jejuni with the Mucosal Surface.
|
|---|---|
| Chapter number | 12 |
| Book title |
Campylobacter jejuni
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6536-6_12 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6534-2, 978-1-4939-6536-6
|
| Authors |
Marguerite Clyne, Gina Duggan, Ciara Dunne, Brendan Dolan, Luis Alverez, Billy Bourke, Luis Alvarez, Clyne, Marguerite, Duggan, Gina, Dunne, Ciara, Dolan, Brendan, Alvarez, Luis, Bourke, Billy |
| Editors |
James Butcher, Alain Stintzi |
| Abstract |
Mucosal colonization and overcoming the mucosal barrier are essential steps in the establishment of infection by Campylobacter jejuni. The interaction between C. jejuni and host cells, including binding and invasion, is thought to be the key virulence factor important for pathogenesis of C. jejuni infections in animals or humans. The intestinal mucosal barrier is composed of a polarized epithelium covered by a thick adherent mucus gel layer. There is a requirement for cell culture assays of infection to accurately represent the in vivo mucosal surface. In this chapter, we describe the use of a number of cell culture models and the use of polarized in vitro organ culture to examine the interaction of C. jejuni with mucosal surfaces. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 9 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 2 | 22% |
| Student > Master | 2 | 22% |
| Student > Postgraduate | 1 | 11% |
| Researcher | 1 | 11% |
| Unspecified | 1 | 11% |
| Other | 0 | 0% |
| Unknown | 2 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 2 | 22% |
| Environmental Science | 1 | 11% |
| Unspecified | 1 | 11% |
| Agricultural and Biological Sciences | 1 | 11% |
| Engineering | 1 | 11% |
| Other | 0 | 0% |
| Unknown | 3 | 33% |