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Mendeley readers
| Chapter title |
Methods to Assess the Direct Interaction of C. jejuni with Mucins.
|
|---|---|
| Chapter number | 10 |
| Book title |
Campylobacter jejuni
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6536-6_10 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6534-2, 978-1-4939-6536-6
|
| Authors |
Marguerite Clyne, Gina Duggan, Julie Naughton, Billy Bourke, Clyne, Marguerite, Duggan, Gina, Naughton, Julie, Bourke, Billy |
| Editors |
James Butcher, Alain Stintzi |
| Abstract |
Studies of the interaction of bacteria with mucus-secreting cells can be complemented at a more mechanistic level by exploring the interaction of bacteria with purified mucins. Here we describe a far Western blotting approach to show how C. jejuni proteins separated by SDS PAGE and transferred to a membrane or slot blotted directly onto a membrane can be probed using biotinylated mucin. In addition we describe the use of novel mucin microarrays to assess bacterial interactions with mucins in a high-throughput manner. |
Mendeley readers
The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 7 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 2 | 29% |
| Student > Master | 1 | 14% |
| Unknown | 4 | 57% |
| Readers by discipline | Count | As % |
|---|---|---|
| Veterinary Science and Veterinary Medicine | 1 | 14% |
| Engineering | 1 | 14% |
| Unknown | 5 | 71% |