Chapter title |
High Resolution Fiber-Fluorescence In Situ Hybridization.
|
---|---|
Chapter number | 14 |
Book title |
Cancer Cytogenetics
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6703-2_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6701-8, 978-1-4939-6703-2
|
Authors |
Christine J. Ye, Henry H. Heng, Ye, Christine J., Heng, Henry H. |
Editors |
Thomas S.K. Wan |
Abstract |
High resolution fiber-Fluorescence in situ hybridization (FISH) is an advanced FISH technology that can effectively bridge the resolution gap between probe hybridizing on DNA molecules and chromosomal regions. Since various types of DNA and chromatin fibers can be generated reflecting different degrees of DNA/chromatin packaging status, fiber-FISH technology has been successfully used in diverse molecular cytogenetic/cytogenomic studies. Following a brief review of this technology, including its major development and increasing applications, typical protocols to generate DNA/chromatin fiber will be described, coupled with rationales, as well as technical tips. These released DNA/chromatin fibers are suitable for an array of cytogenetic/cytogenomic analyses. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 7 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Lecturer > Senior Lecturer | 1 | 14% |
Student > Ph. D. Student | 1 | 14% |
Student > Bachelor | 1 | 14% |
Researcher | 1 | 14% |
Unknown | 3 | 43% |
Readers by discipline | Count | As % |
---|---|---|
Chemistry | 2 | 29% |
Medicine and Dentistry | 1 | 14% |
Biochemistry, Genetics and Molecular Biology | 1 | 14% |
Unknown | 3 | 43% |