| Chapter title |
Isolation and Analysis of Detergent-Resistant Membrane Fractions
|
|---|---|
| Chapter number | 10 |
| Book title |
Lipid Signaling Protocols
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3170-5_10 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3169-9, 978-1-4939-3170-5
|
| Authors |
Massimo Aureli, Sara Grassi, Sandro Sonnino, Alessandro Prinetti |
| Abstract |
The hypothesis that the Golgi apparatus is capable of sorting proteins and sending them to the plasma membrane through "lipid rafts," membrane lipid domains highly enriched in glycosphingolipids, sphingomyelin, ceramide, and cholesterol, was formulated by van Meer and Simons in 1988 and came to a turning point when it was suggested that lipid rafts could be isolated thanks to their resistance to solubilization by some detergents, namely Triton X-100. An incredible number of papers have described the composition and properties of detergent-resistant membrane fractions. However, the use of this method has also raised the fiercest criticisms. In this chapter, we would like to discuss the most relevant methodological aspects related to the preparation of detergent-resistant membrane fractions, and to discuss the importance of discriminating between what is present on a cell membrane and what we can prepare from cell membranes in a laboratory tube. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 64 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 6 | 9% |
| Researcher | 4 | 6% |
| Other | 2 | 3% |
| Student > Bachelor | 2 | 3% |
| Student > Doctoral Student | 2 | 3% |
| Other | 2 | 3% |
| Unknown | 46 | 72% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 5 | 8% |
| Agricultural and Biological Sciences | 4 | 6% |
| Immunology and Microbiology | 2 | 3% |
| Neuroscience | 2 | 3% |
| Chemical Engineering | 1 | 2% |
| Other | 4 | 6% |
| Unknown | 46 | 72% |