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Chapter title |
Determining the Topology of Peroxisomal Proteins Using Protease Protection Assays
|
---|---|
Chapter number | 3 |
Book title |
Peroxisomes
|
Published in |
Methods in molecular biology, April 2017
|
DOI | 10.1007/978-1-4939-6937-1_3 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6935-7, 978-1-4939-6937-1
|
Authors |
Tânia Francisco, Ana F. Dias, Ana G. Pedrosa, Cláudia P. Grou, Tony A. Rodrigues, Jorge E. Azevedo |
Editors |
Michael Schrader |
Abstract |
Protease protection assays are powerful tools to determine the topology of organelle proteins. Their simplicity, together with the fact that they are particularly suited to characterize endogenous proteins, are their major advantages and the reason why these assays have been in use for so many years. Here, we provide a detailed protocol to use with mammalian peroxisomes. Suggestions on how these assays can be controlled, and how to identify some technical pitfalls, are also presented. |
Mendeley readers
The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 16 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 5 | 31% |
Student > Bachelor | 3 | 19% |
Researcher | 3 | 19% |
Student > Doctoral Student | 2 | 13% |
Professor | 1 | 6% |
Other | 1 | 6% |
Unknown | 1 | 6% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 9 | 56% |
Agricultural and Biological Sciences | 3 | 19% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 6% |
Immunology and Microbiology | 1 | 6% |
Chemistry | 1 | 6% |
Other | 0 | 0% |
Unknown | 1 | 6% |