Chapter title |
Analysis of Peroxisomal β-Oxidation During Storage Oil Mobilization in Arabidopsis thaliana Seedlings
|
---|---|
Chapter number | 27 |
Book title |
Peroxisomes
|
Published in |
Methods in molecular biology, April 2017
|
DOI | 10.1007/978-1-4939-6937-1_27 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6935-7, 978-1-4939-6937-1
|
Authors |
Björn Hielscher, Lennart Charton, Tabea Mettler-Altmann, Nicole Linka |
Editors |
Michael Schrader |
Abstract |
Peroxisomal β-oxidation in plants is essential for mobilization of storage oil in seed-oil storing plants, such as Arabidopsis thaliana. In plants, degradation of fatty acids occurs exclusively in peroxisomes via β-oxidation, driving seedling growth and development upon germination. Thus, the determination of storage oil breakdown rates is a useful approach to investigate defects in peroxisomal β-oxidation. Here we describe an acid catalyzed derivatization process of fatty acids representing a fast and efficient procedure to generate high yields of fatty acid methyl esters (FAMEs). The subsequent analysis by gas chromatography coupled to mass spectrometry (GC-MS) allows the quantification of total fatty acid content. The results provide detailed information of the complete storage oil breakdown process via peroxisomal β-oxidation during seedling growth. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 8 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 2 | 25% |
Professor | 1 | 13% |
Unspecified | 1 | 13% |
Researcher | 1 | 13% |
Student > Master | 1 | 13% |
Other | 0 | 0% |
Unknown | 2 | 25% |
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Unspecified | 1 | 13% |
Unknown | 2 | 25% |